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180k microarray slides  (Agilent technologies)


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    Agilent technologies 180k microarray slides
    180k Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    180k microarray slides - by Bioz Stars, 2026-04
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    (a-b) LncRNA microarray expression data from BMDMs incubated in distinct polarizing conditions. A total of 1,251 differentially-expressed lncRNAs were identified between the M(LPS+IFN-γ) and M(IL-4) macrophages. (a) Scatter plots show the variation in lncRNA expression between the M(LPS+IFN-γ) and M(IL-4) macrophages. The values of the X and Y axes in the scatter plot are the averaged normalized values in each group (log2-scaled). (b) Heat maps of lncRNAs expression profiles between the two groups. “Red” indicates high relative expression and “green” indicates low relative expression. One ANOVA test was used for statistical analysis. LncRNA with expression fold change > 2 and with FDR-adiusted P value < 0.05 was considered statistically significant. (c) Confirmation of the differential expression of lncRNAs by RT-qPCR. Four differentially expressed lncRNAs were validated by RT-qPCR. The y-axis represents the log2-transformed median fold-change in expression. (d) LncRNA Dnmt3aos locates on the Dnmt3a opposite strand on mouse chromosome 12. (e) RT-qPCR detection of Dnmt3aos level in cellular fractions from BMDMs. U6 and GAPDH were the nuclear and cytoplasmic controls, respectively. Data were expressed as the means ± SD of three independent experiments. (f) Dnmt3aos expression in BMDMs detected by RNA-FISH. Scale bar, 20 mm.

    Journal: bioRxiv

    Article Title: LncRNA Dnmt3aos regulates Dnmt3a expression leading to aberrant DNA methylation in macrophage polarization

    doi: 10.1101/514307

    Figure Lengend Snippet: (a-b) LncRNA microarray expression data from BMDMs incubated in distinct polarizing conditions. A total of 1,251 differentially-expressed lncRNAs were identified between the M(LPS+IFN-γ) and M(IL-4) macrophages. (a) Scatter plots show the variation in lncRNA expression between the M(LPS+IFN-γ) and M(IL-4) macrophages. The values of the X and Y axes in the scatter plot are the averaged normalized values in each group (log2-scaled). (b) Heat maps of lncRNAs expression profiles between the two groups. “Red” indicates high relative expression and “green” indicates low relative expression. One ANOVA test was used for statistical analysis. LncRNA with expression fold change > 2 and with FDR-adiusted P value < 0.05 was considered statistically significant. (c) Confirmation of the differential expression of lncRNAs by RT-qPCR. Four differentially expressed lncRNAs were validated by RT-qPCR. The y-axis represents the log2-transformed median fold-change in expression. (d) LncRNA Dnmt3aos locates on the Dnmt3a opposite strand on mouse chromosome 12. (e) RT-qPCR detection of Dnmt3aos level in cellular fractions from BMDMs. U6 and GAPDH were the nuclear and cytoplasmic controls, respectively. Data were expressed as the means ± SD of three independent experiments. (f) Dnmt3aos expression in BMDMs detected by RNA-FISH. Scale bar, 20 mm.

    Article Snippet: Each SBC mouse (4*180K) LncRNA microarray slide (Agilent Technologies Inc.) was hybridized with 1.65 μg Cy3-labeled cRNA using a gene expression hybridization kit (Agilent Technologies, Inc.).

    Techniques: Microarray, Expressing, Incubation, Quantitative RT-PCR, Transformation Assay